ABSTRACT
TP53 gene encoding polymorphisms is a risk allele in terms of carcinogenesis. Here, we studied the risk assessment and association of TP53 to understand the cancer biology and behaviour in cervical cancer patients and possible anticancer drug development interfering with p53 protein production. TP53 gene encodes a central protein of apoptosis pathway p53 and its allelic variant has been postulated to play a vital role in carcinogenesis in addition to a variety of neurodegenerative disorders. We undertook a case control study, to examine the possible association of TP53 gene codon 72 polymorphism in leukocyte DNA from a total of 200 subjects (100 controls and 100 cases). TP53 codon Arg72Pro (rs1042522) genotype was identified using allele specific PCR and RFLP with statistical analysis using Vassar Stats (online). In Chhattisgarh population, individuals with GC and GG genotypes of TP53 gene codon 72 polymorphism has a significantly higher risk of cervical cancer (OR=6.36, 95%CI=2.8-14.03 and OR=7.42, 95%CI=3.5-15.9) as compared to CC genotype (OR=1) which was taken as reference. The 'G' allele was found to confer a significant risk of cervical cancer (OR= 3.69, 95%CI= 2.40-5.5) compared to 'C' allele. The present case control study demonstrated the prevalence of the Arg/Arg (GG) genotype in women with cervical cancer among Chhattisgarh population.
ABSTRACT
Background: Sexually transmitted infections (STI) are a major public health, social, and economic problem leading to morbidity, mortality and stigma. This study was conducted to determine the relationship between the STIs and demographic factors. In Indian society, discussing STDs is considered taboo; where ethics and social factors give rise to many obstacles. This study is designed to explore the conception and knowledge about protection against STDs within the patient community. In the present study we aim to study the association of various socioeconomic variables; and the knowledge and awareness level of STI/STDs. Methods: The convenience sample was taken from the adult patients attending and consenting to participate in the study during April- June 2018, using a semi-structured Performa. Collected data was analysed using SPSS soft ware. Results: Age, Education, and income were associated with the awareness about the STI/STD awareness. Conclusions: Socio-economic factors have an association with STI/STD awareness, and hence carry public health importance.
ABSTRACT
A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 μm in size). It grew at temperatures ranging from 4 to 30°C, with a growth optimum at 28°C. It exhibited tolerance to a wide pH range (5-10; optimum 8.0) and salt concentrations up to 6 percent (wt/vol). Although it was sensitive to Rifampicin (R 20 μg mi-1), Gentamicin (G 3 μg mi-1), and Streptomycin (S 5 μg mi-1), it showed resistance to higher concentrations of Ampicillin (A 500 μg mi-1), Penicillin (P 300 μg mi-1), Polymixin B sulphate (Pb 100 μg mi-1) and Chloramphenicol (C 200 μg mi-1). The 16S rRNA sequence analysis revealed maximum identity with Pseudomonas lurida. The bacterium produced indole Acetic Acid (IAA) and solubilizes phosphate at 4, 15 and 28°C. It also retained its ability to produce rhamnolipids and siderophores at 15°C. Seed bacterization with the isolate enhanced the germination, shoot and root lengths of thirty-day-old wheat seedlings by 19.2, 30.0 & 22.9 percent respectively, as compared to the un-inoculated controls.